19康复患者中获得极其高效的人单克隆抗体
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19康复患者中获得极其高效的人单克隆抗体

文章来源:葡京官方平台    时间:2021-02-25

  

本期文章:《细胞》:Online/在线发表

意大利单克隆抗体发现实验室Rino Rappuoli研究团队从COVID-19康复患者中获得了极其高效的人单克隆抗体。2021年2月23日,《细胞》杂志在线发表了这项成果。

通过单细胞分选来自14个COVID-19康复患者的4,277个SARS-CoV-2突刺蛋白特异性记忆B细胞,研究人员鉴定出453种中和抗体。最有效的中和抗体识别刺突蛋白受体结合结构域,其次是识别S1结构域、刺突蛋白三聚体和S2亚基的抗体。它们中只有1.4%的中和力为1-10 ng/mL。

最有效的单克隆抗体经过工程设计,可降低抗体依赖性增强的风险并延长半衰期,并且可中和真正的野生型病毒和含有D614G、E484K和N501Y取代的新兴变体。在不存在Fc功能的情况下,研究人员分别在0.25和4 mg/kg下观察到仓鼠模型中的预防和治疗功效。 

据悉,人单克隆抗体是安全的、可用于预防和治疗的工具,当前需要快速开发来帮助由COVID-19大流行引起的大规模健康和经济破坏。

附:英文原文

Title: Extremely potent human monoclonal antibodies from COVID-19 convalescent patients

Author: Emanuele Andreano, Emanuele Nicastri, Ida Paciello, Piero Pileri, Noemi Manganaro, Giulia Piccini, Alessandro Manenti, Elisa Pantano, Anna Kabanova, Marco Troisi, Fabiola Vacca, Dario Cardamone, Concetta De Santi, Jonathan L. Torres, Gabriel Ozorowski, Linda Benincasa, Hyesun Jang, Cecilia Di Genova, Lorenzo Depau, Jlenia Brunetti, Chiara Agrati, Maria Rosaria Capobianchi, Concetta Castilletti, Arianna Emiliozzi, Massimiliano Fabbiani, Francesca Montagnani, Luisa Bracci, Giuseppe Sautto, Ted M. Ross, Emanuele Montomoli, Nigel Temperton, Andrew B. Ward, Claudia Sala, Giuseppe Ippolito, Rino Rappuoli

Issue&Volume: 2021-02-23

Abstract: Human monoclonal antibodies are safe, preventive and therapeutic tools, that can be rapidly developed to help restore the massive health and economic disruption caused by the coronavirus disease 2019 (COVID-19) pandemic. By single cell sorting 4,277 SARS-CoV-2 spike protein specific memory B cells from 14 COVID-19 survivors, 453 neutralizing antibodies were identified. The most potent neutralizing antibodies recognized the spike protein receptor binding domain, followed in potency by antibodies that recognize the S1 domain, the spike protein trimer and the S2 subunit. Only 1.4% of them neutralized the authentic virus with a potency of 1-10 ng/mL. The most potent monoclonal antibody, engineered to reduce the risk of antibody dependent enhancement and prolong half-life, neutralized the authentic wild type virus and emerging variants containing D614G, E484K and N501Y substitutions. Prophylactic and therapeutic efficacy in the hamster model was observed at 0.25 and 4 mg/kg respectively in absence of Fc-functions.

DOI: 10.1016/j.cell.2021.02.035

Source: https://www.cell.com/cell/fulltext/S0092-8674(21)00224-5

期刊信息

Cell:《细胞》,创刊于1974年。隶属于细胞出版社,最新IF:36.216

官方网址:https://www.cell.com/

投稿链接:https://www.editorialmanager.com/cell/default.aspx

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