2 501Y.V2变体没有更高的传染性,但能够免疫逃逸
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2 501Y.V2变体没有更高的传染性,但能够免疫逃逸

文章来源:葡京官方平台    时间:2021-02-25

  

本期文章:《细胞》:Online/在线发表

中国食品药品检定研究院Youchun Wang等研究人员合作发现,SARS-CoV-2 501Y.V2变体没有更高的传染性,但能够免疫逃逸。这一研究成果于2021年2月23日在线发表在国际学术期刊《细胞》上。

研究人员使用18种假型病毒进行的实验表明,除了鼠ACE2过表达的细胞外,501Y.V2变体在多种细胞类型中均未赋予感染力增强。在小鼠中,ACE2过表达的细胞观察到感染力的极大增加。值得注意的是,501Y.V2变体对17种中和单克隆抗体中的12种的敏感性极大降低,并且恢复期患者和免疫小鼠血清对这些变体的中和能力也降低了。中和抗性主要是由突刺受体结合域中的E484K和N501Y突变引起的。鼠ACE2过表达细胞中增强的感染性表明501Y.V2变体可能会向小鼠产生溢出效应。

此外,研究人员检测到的501Y.V2变体抗中和性表明,潜在的单克隆抗体和疫苗功效可能受影响。 

据介绍,SARS-CoV-2的501Y.V2变体在突刺蛋白中含有多个突变,目前在南非占主导地位,并迅速传播到其他国家。

附:英文原文

Title: No higher infectivity but immune escape of SARS-CoV-2 501Y.V2 variants

Author: Qianqian Li, Jianhui Nie, Jiajing Wu, Li Zhang, Ruxia Ding, Haixin Wang, Yue Zhang, Tao Li, Shuo Liu, Mengyi Zhang, Chenyan Zhao, Huan Liu, Lingling Nie, Haiyang Qin, Meng Wang, Qiong Lu, Xiaoyu Li, Junkai Liu, Haoyu Liang, Yi Shi, Yuelei Shen, Liangzhi Xie, Linqi Zhang, Xiaowang Qu, Wenbo Xu, Weijin Huang, Youchun Wang

Issue&Volume: 2021-02-23

Abstract: The 501Y.V2 variants of SARS-CoV-2 containing multiple mutations in Spike are now dominant in South Africa and are rapidly spreading to other countries. Here, experiments with 18 pseudotyped viruses showed that the 501Y.V2 variants do not confer increased infectivity in multiple cell types except for murine ACE2-overexpressing cells, where a substantial increase in infectivity was observed. Notably, the susceptibility of the 501Y.V2 variants to 12 of 17 neutralizing monoclonal antibodies was substantially diminished, and the neutralization ability of the sera from convalescent patients and immunized mice was also reduced for these variants. The neutralization resistance was mainly caused by E484K and N501Y mutations in the receptor-binding domain of Spike. The enhanced infectivity in murine ACE2-overexpressing cells suggests the possibility of spillover of the 501Y.V2 variants to mice. Moreover, the neutralization resistance we detected for the 501Y.V2 variants suggests the potential for compromised efficacy of monoclonal antibodies and vaccines.

DOI: 10.1016/j.cell.2021.02.042

Source: https://www.cell.com/cell/fulltext/S0092-8674(21)00231-2

期刊信息

Cell:《细胞》,创刊于1974年。隶属于细胞出版社,最新IF:36.216

官方网址:https://www.cell.com/

投稿链接:https://www.editorialmanager.com/cell/default.aspx

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